Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: GNM-45-1049

[Submit comment/correction]

Reference	GNM-45-1049
Title	An isoleucine to leucine mutation in acetyl-CoA carboxylase confers herbicide resistance in wild oat
Journal	Genome
Year	2002
Volume	45
Pages	1049-1056
Author	Christoffers MJ Berg ML Messersmith CG
Abstract	Summary: Wild oat (Avena fatua L.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase; EC 6.4.1.2) represent an increasingly important weed control problem. The objective of this study was to determine the ACCase mutation responsible for herbicide resistance in a well-studied wild oat biotype (UM1). A 2039-bp region encompassing the carboxybiotin and acetyl-CoA binding domains of multifunctional plastidic ACCase was analyzed. DNA sequences representing three plastidic ACCase gene loci were isolated from both the resistant UM1 and a herbicide-susceptible biotype, consistent with the hexaploid nature of wild oat. Only one nonsynonymous point mutation was found among the resistant wild oat sequences, inferring an isoleucine to leucine substitution. The position of this substitution corresponds to residue 1769 of wheat (Triticum aestivum L.) plastidic ACCase (GenBank accession No. AF029895). Analysis of an F2 population derived from a cross between a herbicide-resistant and a susceptible biotype confirmed co-segregation of herbicide resistance with the mutated ACCase. We conclude that the isoleucine to leucine mutation is responsible for herbicide resistance in UM1 wild oat based on a comparison of the substitution site across species and ACCase types. While isoleucine is conserved among plastidic ACCases of herbicide-susceptible grasses, leucine is found in plastidic and cytosolic forms of multifunctional herbicide-resistant ACCase
External Databases	Pubmed: 12502249
Keyword	[ Hide all but 1 of 35 ] acetyl-coa carboxylase aryloxyphenoxypropionate avena avena fatua biotype canada carboxyltransferase domain coenzyme-a carboxylase control cross cross-resistance cyclohexanedione herbicides cyclotella-cryptica dna f-2 population gene herbicide resistance herbicides hexaploid inhibitor resistance locus molecular-cloning mutation oat region resistance resistant sequence site triticum triticum aestivum weed weed control wild wild oat

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.