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GrainGenes Reference Report: GNM-45-977

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Reference	GNM-45-977
Title	Direct capture and cloning of receptor kinase and peroxidase genes from genomic DNA
Journal	Genome
Year	2002
Volume	45
Pages	977-983
Author	Cheng DW Armstrong KC
Abstract	Summary: A direct DNA capture and cloning procedure with magnetic bead separation was used to isolate receptor kinase like and peroxidase genes from oat (Avena sativa) and wheat (Triticum aestivum L.) genomic DNA, respectively. In this procedure, the digoxigenin-labeled probe DNA and target genomic DNA fragments were mixed, denatured, and hybridized. The double-helix complexes formed were captured with anti-digoxigenin immunoglobulin-coated magnetic beads and then cloned into either the lambdaBlueSTAR or pUC18 vector. The effectiveness of this procedure was demonstrated by using two specific DNA probes to capture receptor-like kinase genes and surrounding sequences from oat genomic DNA and a peroxidase gene from wheat genomic DNA
External Databases	Pubmed: 12416632
Keyword	[ Hide all but 1 of 20 ] artificial chromosome library avena sativa canada cloning dna enrichment fragments gene magnetic beads oat peroxidase purification receptor rust-resistance genes selection separation sequence triplex affinity capture triticum aestivum vector

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