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GrainGenes Reference Report: GNM-45-719

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Reference	GNM-45-719
Title	Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers
Journal	Genome
Year	2002
Volume	45
Pages	719-727
Author	Zhou WC Kolb FL Bai GH Shaner G Domier LL
Abstract	Summary: Three chromosomal regions associated with scab resistance were detected in a common cultivar, Ning7840, by microsatellite and AFLP analysis. Six microsatellites on chromosome 3BS, Xgwm389, Xgwm533, Xbarc147, Xgwm493, Xbarc102, and Xbarc131, were integrated into an amplified fragment length polymorphism (AFLP) linkage group containing a major quantitative trait locus (QTL) for scab resistance in a mapping population of 133 recombinant inbred lines (RILs) derived from 'Ning7840' x 'Clark'. Based on single-factor analysis of variance of scab infection data from four experiments, Xgwm533 and Xbarc147 were the two microsatellite markers most tightly associated with the major scab resistance QTL. Interval analysis based on the integrated map of AFLP and microsatellite markers showed that the major QTL was located in a chromosome region about 8 cM in length around Xgwm533 and Xbarc147. Based on mapping of six microsatellite markers on eight 3BS deletion lines, the major QTL was located distal to breakage point 3BS-8. In total, 18 microsatellites were physically located on different subarm regions on 3BS. Two microsatellites, Xgwm120 and Xgwm614, were significantly associated with QTL for scab resistance on chromosome 2BL and 2AS, respectively. The resistance alleles on 3BS, 2BL, and 2AS were all derived from 'Ning7840'. Significant interaction between the major QTL on 3BS and the QTL on 2BL was detected based on microsatellite markers linked to them. Using these microsatellite markers would facilitate marker-assisted selection to improve scab resistance in wheat
External Databases	Pubmed: 12175075
QTL	QFhs.ndsu.3B
Keyword	[ Hide all but 1 of 43 ] aflp aflp analysis amplified fragment length polymorphism analysis of variance bread wheat breakage canada chromosome chromosome-3b common wheat cultivar deletion fusarium head blight graminearum infection integrated map interval length polymorphism length-polymorphism linkage linkage group locus map mapping physical mapping physical maps polymorphism population qtl quantitative trait quantitative trait locus recombinant region resistance rflp rich regions ril rils scab scab resistance selection trait variance

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