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GrainGenes Reference Report: GNM-45-706

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Reference	GNM-45-706
Title	Genomic targeting and high-resolution mapping of the domestication gene Q in wheat
Journal	Genome
Year	2002
Volume	45
Pages	706-718
Author	Faris JD Gill BS
Abstract	Summary: The Q locus is largely responsible for the domestication of bread wheat. Q confers the free-threshing character of the spike and influences other important agronomic traits. Using chromosome deletion lines, Q was placed on the physical map within a submicroscopic segment of the long arm of chromosome 5A. We targeted markers to the segment by comparative mapping of anonymous RFLP clones, AFLP, and mRNA differential display analysis of deletion lines 5AL-7 and -23, which have deletion breakpoints that flank the Q locus. Differentially expressed sequences detected fragments at various loci on group 5 chromosomes suggesting that Q may be a regulatory gene. We identified 18 markers within the Q gene deletion interval and used them to construct a genetic linkage map of the region in F2 populations derived from chromosome 5A disomic substitution lines. The genetic map corresponding to the deletion segment was 20-cM long, and we identified markers as close as 0.7 cM to the Q gene. An estimate of base pairs per centimorgan within the region is 250 kb/cM, an 18-fold increase in recombination compared with the genomic average. Genomic targeting and high-density mapping provide a basis for the map-based cloning of the Q gene
External Databases	Pubmed: 12175074
Gene	Q (Triticum)
Map Data	5AL, CS x CS-TDIC DS5A
Probe	KSUQ45 KSUQ34 KSUQ16 KSUQ62
Keyword	[ Hide all but 1 of 65 ] 50 5a aegilops speltoides aflp agronomic trait agronomic traits base bread breakpoints canada chromosome chromosome 5a chromosome deletion line chromosome-5a cloning common wheat comparative mapping deletion density differential display disomic substitution disomic substitution lines domestication expressed sequences f-2 population fragments gametocidal genes gene genetic linkage genetic linkage map genetic map genetic-linkage group-5 high-density high-resolution mapping increase interval linkage linkage map linkage-map long arm map map-based cloning mapping messenger rna mrna mrna differential display physical map physical mapping positional cloning q gene recombination region regulatory regulatory gene rflp rich regions segment sequence spike substitution substitution line targeting trait triticum aestivum
Image	5AL_Q_map

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