Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: GNM-45-421

[Submit comment/correction]

Reference	GNM-45-421
Title	Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants
Journal	Genome
Year	2000
Pages	421-430
Author	Pellegrineschi A [ Show all 9 ]
Abstract	Summary: The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A number of factors influencing the transformation were examined, such as the ability to produce embryogenic callus, regeneration in selection medium, and overall transformation performance. Of the 129 genotypes evaluated, eight demonstrated transformation efficiencies above 60% (60 independent transgenic events per 100 immature embryos bombarded). Among the eight genotypes identified, we studied agronomic characteristics such as earliness to identify the most adaptable line(s) for different lab conditions. 'Bobwhite' SH 98 26 was identified as a super-transformable wheat line
Keyword	[ Hide all but 1 of 30 ] abilities agronomic characteristics biolistic biolistic transformation biolistics bombardment callus donor earliness embryo embryogenic callus events fertile fertile transgenic plants genetic transformation mass-production media paper parameters performance plant embryos production regeneration selection system transformation transformation efficiency transgenic triticum aestivum wheat genotypes

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.