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GrainGenes Reference Report: GNM-45-198

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Reference	GNM-45-198
Title	Rapid verification of wheat-Hordeum introgressions by direct staining of SCAR, STS, and SSR amplicons
Journal	Genome
Year	2002
Volume	45
Pages	198-203
Author	Hernandez P [ Show all 6 ]
Abstract	Summary: A range of single tagged site (STS), simple sequence repeat (SSR), and sequence-characterized amplified region (SCAR) markers were screened for their utility in detecting Hordeum vulgare and H. chilense chromosomes in a wheat background. PCR conditions were optimized for specific amplification of the targeted sequences and to avoid cross-species amplification. Two H. vulgare derived STSs, six H. vulgare derived SSRs, and nine H. chilense derived SCARs were usable for the detection of five H. vulgare and three H. chilense chromosomes by direct ethidium bromide staining of the PCR products in test tubes, avoiding the more costly and time-consuming DNA electrophoresis step. The practical application of the method is illustrated by the identification of a monotelosomic substitution of H. vulgare chromosome 6HS in tritordeum and a monosomic addition of H. chilense chromosome 6H(ch) in durum wheat
Keyword	[ Hide all but 1 of 41 ] addition amplicons amplification application barley canada chilense chromosome cross-species amplification detection dna durum durum wheat electrophoresis ethidium bromide gish hordeum hordeum chilense hordeum vulgare introgression marker marker-assisted selection method pcr pcr markers pcr products products region repeat scar sequence simple sequence repeat site specific amplification ssr sts substitution tritordeum tube utility verification

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