Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: GNM-45-116

[Submit comment/correction]

Reference	GNM-45-116
Title	A DNA mismatch repair gene links to the Ph2 locus in wheat
Journal	Genome
Year	2002
Volume	45
Pages	116-124
Author	Dong CM Whitford R Langridge P
Abstract	Summary: DNA mismatch repair is an essential system for maintaining genetic stability in bacteria and higher eukaryotes. Based on the conserved regions of the bacterial MutS gene and its homologues in yeast and human, a wheat cDNA homologue of MSH6, designated TaMSH7, was isolated by RT-PCR. The deduced amino acid sequence of TaMSH7 shows conserved domains characteristic of other MSH6 genes, with highest similarity to maize MSH7 and Arabidopsis MSH7. TaMSH7 is expressed in meristem tissue associated with a high level of mitotic and meiotic activity, with maximum expression in the reproductive organs of young flower spikes. TaMSH7 is located on the short arms of chromosomes 3A, 3B, and 3D and has been mapped within barley chromosome 3HS. The copy on 3DS is located within the region deleted in the wheat mutant ph2a, which shows altered recombination frequency in the interspecific hybrids. The relationship between the ph2a mutant and TaMSH7 gene function is discussed
Keyword	[ Hide all but 1 of 61 ] acid amino acid amino acid sequence arabidopsis arabidopsis thaliana arms bacteria barley canada cdna chromosomal location chromosome common wheat conserved domains conserved region copies crossing-over dna dna mismatch repair gene domain eukaryotes flower frequencies function gene genetic stability high-level homeologous recombination human hybrid interspecific hybrid its link locus maize maximum meiosis meristem mismatch repair msh2 mutant muts homolog p ph2 ph2 locus protein recombination recombination frequency region repair rt-pcr saccharomyces cerevisiae sequence short arms similarities spike stability system tissue wheat mutant yeast

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.