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GrainGenes Reference Report: GNM-44-1122

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Reference	GNM-44-1122
Title	Introgression of rye chromatin on chromosome 2D in the Portuguese wheat landrace 'Barbela'
Journal	Genome
Year	2001
Volume	44
Pages	1122-1128
Author	Ribeiro-Carvalho C Guedes-Pinto H Heslop-Harrison JS Schwarzacher T
Abstract	Summary: The old Portuguese wheat landrace aggregate known as 'Barbela' shows good productivity under the low-fertility conditions often associated with acid soils. The use of genomic rye DNA, in combination with 45S rDNA and the repetitive sequences dpTa1 and pSc119.2 as probes, in two sequential in situ hybridization steps enabled the identification of all chromosomes in the 'Barbela' wheat lines and the detection of the introgression of rye-origin chromatin onto wheat chromosome arm 2DL in two of the lines. Amplification of microsatellite loci using published primer pairs showed that the distal segment of wheat chromosome 2DL, which was involved in the rye translocation, was deleted. The identification and characterization of small recombinant chromosome segments in wheat-rye lines may allow their use in plant breeding programmes. Their presence in farmer-maintained material demonstrates the importance of maintaining, characterizing, and collecting landrace material before valuable genetic combinations are lost as uniform commercial crops are introduced
Keyword	[ Hide all but 1 of 54 ] 2d 45s rdna acid acid soil acid soils alien chromosomes amplification biodiversity bread wheat breeding breeding programme canada chromatin chromosome chromosome 2d chromosome arm chromosome segments common wheat crop d genome detection dna dna sequences genetic diversity hexaploid wheat hybridization in situ in situ hybridization insitu hybridization introgression landrace loci marker selection microsatellite microsatellite loci microsatellite marker old plant breeding primer probes productivity rdna recombination repetitive sequence rye rye translocation segment sequence situ hybridization soil soils translocation triticeae wheat landrace

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