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GrainGenes Reference Report: GNM-44-1031

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Reference	GNM-44-1031
Title	Construction of a YAC library from barley cultivar Franka and identification of YAC-derived markers linked to the Rh2 gene conferring resistance to scald (Rhynchosporium secalis)
Journal	Genome
Year	2000
Pages	1031-1040
Author	Schmidt D [ Show all 7 ]
Abstract	Summary: The Rh2 resistance gene of barley (Hordeum vulgare) confers resistance against the scald pathogen (Rhynchosporium secalis). A high-resolution genetic map of the Rh2 region on chromosome 1 (7H) was established by the use of molecular markers. Tightly linked markers from this region were used to screen existing and a newly constructed yeast artificial chromosome (YAC) library of barley cv. Franka composed of 45 000 clones representing approximately two genome equivalents. Corresponding YAC clones were identified for most markers, indicating that the combined YAC library has good representation of the barley genome. The contiguous sets of YAC clones with the most tightly linked molecular markers represent entry points for map-based cloning of this resistance gene
Keyword	[ Hide all but 1 of 39 ] 45 artificial chromosome barley barley genome chromosome cloning conferring resistance confers resistance cultivar disease resistance dna libraries entry gene genetic map genetic mapping genetic marker genetic resistance genome genome equivalents hordeum linkage linked molecular markers map map-based cloning molecular marker pathogen region representation resistance resistance gene rhynchosporium rhynchosporium secalis scald screen yac yac clones yac library yeast yeast artificial chromosomes

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