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GrainGenes Reference Report: GNM-44-883

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Reference	GNM-44-883
Title	Genetic and physical characterization of chromosome 4DL in wheat
Journal	Genome
Year	2001
Volume	44
Pages	883-892
Author	Milla MAR Gustafson JP
Abstract	The long arm of chromosome 4D in wheat (Triticum aestivum L.) has been shown in previous studies to harbor genes of agronomic importance. A major dominant gene conferring Aluminum (Al) tolerance (Alt2 in 'Chinese Spring' and Alt(BH) in 'BH 1146'), and the Kna1 locus controlling the K(+)/Na(+) discrimination in saline environments have been mapped to this chromosome arm. However, accurate information on the genetic and physical location of markers related to any of these genes is not available and would be useful for map-based cloning and marker-assisted plant breeding. In the present study, using a population of 91 recombinant inbred lines segregating for Al tolerance, we provide a more extensive genetic linkage map of the chromosome arm 4DL based on RFLP, SSR, and AFLP markers, delimiting the Alt(BH) gene to a 5.9-cM interval between markers Xgdm125 and Xpsr914. In addition, utilizing a set of wheat deletion lines for chromosome arm 4DL, the Alt(BH) gene was physically mapped to the distal region of the chromosome, between deletion breakpoints 0.70 and 0.86, where the kilobase/centimorgan ratio is assumed to be low, making the map-based cloning of the gene a more realistic goal. The polymorphism rates in chromosome arm 4DL for the different types of markers used were extremely low, as confirmed by the physical mapping of AFLPs. Finally, analysis of 1 Mb of contiguous sequence of Arabidopsis chromosome 5 flanking the gene homologous to the BCD1230 clone (a cosegregating marker in our population coding for a ribulose-5-phosphate-3-epimerase gene), revealed a previously identified region of stress-related and disease-resistance genes. This could explain the collinearity observed in comparative mapping studies among different species and the low level of polymorphism detected in the chromosome arm 4DL in hexaploid wheat
External Databases	https://doi.org/10.1139/g01-089
Gene	Sr9 (Triticum)
Keyword	[ Hide all but 1 of 59 ] addition aflp aflp marker agronomic importance al aluminum aluminum tolerance gene arabidopsis bread wheat breeding canada chinese chinese spring chromosome chromosome arm clone cloning comparative mapping deletion deletion line discrimination disease resistance disease resistance gene dominant dominant gene environment gene genetic linkage genetic linkage map hexaploid hexaploid wheat inbred lines interval k+/na+ discrimination linkage linkage map locus long arm map map-based cloning mapping marker oct physical location physical mapping plant breeding polymorphism population rate recombinant inbred line region rflp rflp marker sequence spring ssr tolerance triticum triticum aestivum

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