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GrainGenes Reference Report: GNM-44-529

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Reference	GNM-44-529
Title	The specific isolation of complete 5S rDNA units from chromosome 1A of hexaploid, tetraploid, and diploid wheat species using PCR with head-to-head oriented primers
Journal	Genome
Year	2001
Volume	44
Pages	529-538
Author	Van Campenhout S Vander Stappen J Volckaert G
Abstract	Summary: The presence of 5S rDNA units on chromosome 1A of Triticum aestivum was shown by the development of a specific PCR test, using head-to-head oriented primers. This primer set allowed the amplification of complete 5S DNA units and was used to isolate 5S-Rrna-A1 sequences from polyploid and diploid wheat species. Multiple-alignment and parsimony analyses of the 132 sequences divided the sequences into four types. The isolates from T. aestivum and the tetraploid species (T. dicoccoides, T. dicoccum, T. durum, T. araraticum, and T. timopheevi) were all of one type, which was shown to be closely related to the type mainly characteristic for T. urartu. The other two types were isolated exclusively from the diploid species T. monococcum, T. aegilopoides, T. thaoudar, and T. sinskajae and the hexaploid species T. zhukovski. Triticum monococcum was the only species for which representatives of each of the four sequence types were found to be present. Further, we discuss the possible multicluster arrangement of the 5S-Rrna-A1 array
Keyword	[ Hide all but 1 of 35 ] 5s 5s rdna 5s ribosomal rna locus a genome aegilopoides amplification arrangement bread wheat canada chromosome chromosome-1a dicoccoides diploid wheat durum evolution genetic hexaploid isolate isolation multigene family organization parsimony pcr pcr markers polymorphism polyploid primer rdna rdna units ribosomal rna gene sequence triticum triticum aestivum triticum monococcum wheat species

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