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GrainGenes Reference Report: GNM-44-401

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Reference	GNM-44-401
Title	Physical mapping of restriction fragment length polymorphism (RFLP) markers in homoeologous groups 1 and 3 chromosomes of wheat by in situ hybridization
Journal	Genome
Year	2001
Volume	44
Pages	401-412
Author	Ma XF Ross K Gustafson JP
Abstract	Summary: Using wheat ditelosomic lines and in situ hybridization of biotin-labelled DNA probes, 18 restriction fragment length polymorphism (RFLP) markers were physically located on homoeologous groups 1 and 3 chromosomes of wheat. Most of the markers hybridized to chromosome arms in a physical order concordant with the genetic maps. A majority of the markers studied were clustered in non-C-banded, distal euchromatic areas, indicating the presence of recombination hot spots and cold spots in those regions. However, on 1BS the markers were well dispersed, which could be due to the abundance of heterochromatin throughout the arm. An inversion between Xpsr653 and Xpsr953 was observed on 1AL. One new Xpsr688 locus, approximately 20-26% from the centromere, was found on 1AS and 1BS. The physical location of Xpsr170 on group 3 chromosomes probably represents an alternative to the loci on the genetic map. Finally, Xpsr313 was mapped to two physical loci on 1DL. Five markers were located to bins consistent with the deletion-based physical maps
Gene	Sr31 (Triticum) Sr9 (Triticum)
Keyword	[ Hide all but 1 of 47 ] 1b arms canada centromere chromosome cold deletion lines ditelosomic ditelosomic line dna dna probes dna sequences field gel-electrophoresis fragment length polymorphism gene-rich regions genetic genetic map genome heterochromatin hordeum vulgare hybridization in situ in situ hybridization insitu hybridization inversion length polymorphism line linkage map loci locus map mapping maps marker molecular biology physical location physical map physical mapping polymorphism probes recombination region restriction rflp situ hybridization spot triticum aestivum

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