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GrainGenes Reference Report: GNM-44-375

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Reference	GNM-44-375
Title	Resistance gene analogs in barley and their relationship to rust resistance genes
Journal	Genome
Year	2001
Volume	44
Pages	375-381
Author	Collins N Park R Spielmeyer W Ellis J Pryor AJ
Abstract	Summary: Regions of amino acid conservation in the NBS domain of NBS-LRR resistance proteins facilitated the PCR isolation of eight resistance gene analog (RGA) sequences from genomic DNA of rice, barley, and Aegilops tauschii. These clones and other RGAs previously isolated from maize, rice, and wheat were assigned to 13 classes by DNA-sequence comparison and by their patterns of hybridisation to restricted barley DNA. Using a doubled-haploid mapping population, probes from 12 RGA classes were used to map 17 loci in the barley genome. Many of these probes have been used for mapping in wheat, and the collective data indicate that the positions of orthologous RGAs are conserved between barley and wheat. RGA loci were identified in the vicinity of barley leaf rust resistance loci Rph4, Rph7, and Rph10. Recombinants were identified between RGA loci and Rph7 and Rph10, while a cluster of RGA sequences detected by probe 5.2 cosegregated with Rph4 in 55 F2 lines
Keyword	[ Hide all but 1 of 56 ] acid aegilops aegilops tauschii amino acid analogs arabidopsis thaliana barley barley genome barley leaves canada clone cluster conservation disease resistance dna dna sequence domain doubled haploid gene gene analogs genetic genome genomic dna homolog hordeum vulgare isolation leaf rust leaf rust resistance line loci maize map mapping molecular biology molecular characterization nbs lrr nucleotide binding pattern pcr population position probes protein puccinia region resistance resistance gene resistance gene analog rga rice rust rust resistance rust resistance gene rust resistance genes sequence tauschii

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