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GrainGenes Reference Report: GNM-44-345

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Reference	GNM-44-345
Title	Isolation of a chromosomally engineered durum wheat line carrying the Aegilops ventricosa Pch1 gene for resistance to eyespot
Journal	Genome
Year	2001
Volume	44
Pages	345-349
Author	Huguet-Robert V [ Show all 8 ]
Abstract	Summary: The chromosome 7D(v) of Aegilops ventricosa (syn. Triticum ventricosum, 2n = 4x = 28, genome D(v)D(v)M(v)M(v)) carries the gene Pch1 for resistance to eyespot. This gene has previously been transferred to chromosome 7D of bread wheat, T. aestivum (2n = 6x = 42, genome AABBDD). To (1) enhance the level of resistance of bread wheat by increasing the copy number of Pch1, and (2) create eyespot-resistant triticales, meiotically stable Pch1-carrying durum lines were selected from the backcross progenies of a cross between Ae. ventricosa and T. durum cv. Creso ph1c (2n = 4x = 28, genome AABB). The Pch1 transfer, likely resulting from homoeologous recombination, was located at the distal position on the long arm of chromosome 7A. The 7A microsatellite marker Xgwm 698 was found closely linked in repulsion to the introgression in the resistant recombination lines, and the endopeptidase allele located on chromosome 7A of cv. Creso ph1c was lost
Keyword	[ Hide all but 1 of 34 ] aegilops aegilops ventricosa backcross bread bread wheat canada chromosome copy number cross crosses durum durum wheat eyespot gene genetic genome homoeologous recombination introgression isolation line long arm mapping marker microsatellite microsatellite marker molecular biology position progeny recombination resistance resistant transfer triticale triticum

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