Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: GNM-44-137

[Submit comment/correction]

Reference	GNM-44-137
Title	Production of durum wheat substitution haploids from durum x maize crosses and their cytological characterization
Journal	Genome
Year	2001
Volume	44
Pages	137-142
Author	Dogramaci-Altuntepe M Jauhar PP
Abstract	Summary: The objective of this study was to investigate the effect of individual durum wheat (Triticum turgidum L.) chromosomes on crossability with maize (Zea mays L.) and to cytologically characterize the haploids recovered. Fourteen 'Langdon' (LDN) D-genome disomic substitution lines, a LDN Ph mutant (Ph1b ph1b), and normal 'Langdon' were pollinated with maize pollen. After pollination, hormonal treatment was given daily for up to 14 days. Haploid embryos were obtained from all lines and were aseptically cultured. From a total of 55358 pollinated florets, 895 embryos were obtained. Only 14 of the embryos germinated and developed into healthy plants. Different substitution lines showed varying degrees of success. The most successful was the substitution 5D(5B) for both embryo formation and haploid plantlet production. These results indicate that the substitution of 5D for 5B confers on durum wheat a greater ability to produce haploids. Fluorescent genomic in situ hybridization (GISH) showed that the substitution haploids consisted of 7 A-genome chromosomes, 6 B-genome chromosomes, and 1 D-genome chromosome. Triticum urartu Tum. genomic DNA was efficient in probing the 7 A-genome chromosomes, although the D-genome chromosome also showed intermediate hybridization. This shows a close affinity between the A genome and D genome. We also elucidated the evolutionary translocation involving the chromosomes 4A and 7B that occurred at the time of evolution of durum wheat. We found that the distal segment translocated from chromosome 7B constitutes about 24% of the long arm of 4A
Keyword	[ Hide all but 1 of 50 ] a genome affinities b genome canada chromosome crossability culture conditions cyclic translocation 4a center dot 5a center dot 7b d genome disomic substitution disomic substitution lines dna durum durum wheat embryo evolution evolutionary fluorescent genomic in situ hybridization (gish) genetic genome genomic dna genomic in situ hybridization gish haploid healthy hexaploid wheat high-frequencies hybridization in situ in situ hybridization long arm maize maize pollen molecular biology mutant ph pollen pollination regeneration segment substitution substitution lines translocation triticum aestivum triticum turgidum triticum urartu turgidum x maize crosses zea zea mays

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.