Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: GNM-44-32

[Submit comment/correction]

Reference	GNM-44-32
Title	A physical map with yeast artificial chromosome (YAC) clones covering 63% of the 12 rice chromosomes
Journal	Genome
Year	2000
Volume	44
Pages	32-37
Author	Saji S [ Show all 12 ]
Abstract	Summary: A new YAC (yeast artificial chromosome) physical map of the 12 rice chromosomes was constructed utilizing the latest molecular linkage map. The 1439 DNA markers on the rice genetic map selected a total of 1892 YACs from a YAC library. A total of 675 distinct YACs were assigned to specific chromosomal locations. In all chromosomes, 297 YAC contigs and 142 YAC islands were formed. The total physical length of these contigs and islands was estimated to 270 Mb which corresponds to approximately 63% of the entire rice genome (430 Mb). Because the physical length of each YAC contig has been measured, we could then estimate the physical distance between genetic markers more precisely than previously. In the course of constructing the new physical map, the DNA markers mapped at 0.0-cM intervals were ordered accurately and the presence of potentially duplicated regions among the chromosomes was detected. The physical map combined with the genetic map will form the basis for elucidation of the rice genome structure, map-based cloning of agronomically important genes, and genome sequencing
Keyword	[ Hide all but 1 of 40 ] artificial chromosome chromosomal location chromosome clone cloning contig distance dna dna marker duplicated regions gene genetic genetic map genetic mapping genetic markers genome genome analysis genome sequencing interval island library linkage linkage map map map-based cloning marker molecular linkage map oryza sativa physical map region rice rice chromosomes rice genome sequencing structure yac yac contig yac library yeast yeast artificial chromosomes

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.