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GrainGenes Reference Report: GNM-43-1045

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Reference	GNM-43-1045
Title	Production and meiotic pairing behaviour of new hybrids of winter wheat (Triticum aestivum) x winter barley (Hordeum vulgare)
Journal	Genome
Year	2000
Volume	43
Pages	1045-1054
Author	Molnar-Lang M Linc G Logojan A Sutka J
Abstract	Summary: New winter wheat (Triticum aestivum L.) x winter barley (Hordeum vulgare L.) hybrids produced using cultivated varieties (wheat 'Martonvasari 9 kr1'(Mv9 kr1) x barley 'Igri', Mv9 kr1 x 'Osnova', 'Asakazekomugi' x 'Manas') were multiplied in tissue culture because of the high degree of sterility and then pollinated with wheat to obtain backcross progenies. Meiotic analysis of the hybrids Mv9 kr1 x 'Igri' and 'Asakazekomugi' x 'Manas' and their in vitro regenerated progenies with the Feulgen method revealed 1.59 chromosome arm associations per cell in both initial hybrids. The number of chromosome arm associations increased after in vitro culture to 4.72 and 2.67, respectively, in the two combinations. According to the genomic in situ hybridization (GISH) analysis, wheat-barley chromosome arm associations made up 3.6% of the total in the initial Mv9 kr1 x 'Igri' hybrid and 6.6% and 16.5% of the total in in vitro regenerated progenies of the 'Asakazekomugi' x 'Manas' and Mv9 kr1 x 'Igri' hybrids, respectively. The demonstration by GISH of wheat-barley chromosome pairing in the hybrids and especially in their in vitro regenerated progenies proves the possibility of producing recombinants between these two genera, and thus of transferring useful characters from barley into wheat. In vitro conditions caused an increase in chromosome arm association frequency in both combinations and in fertility in some regenerants
Keyword	[ Hide all but 1 of 33 ] backcross barley bread wheat canada cell chromosome pairing crossability culture fertility genera genomic in situ hybridization gish hordeum vulgare hybrid hybridization in situ in situ hybridization in vitro in vitro culture intergeneric hybridization meiotic analysis meiotic chromosome pairing meiotic pairing method progeny regenerants sterility tissue tissue culture triticum aestivum winter winter barley winter wheat

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