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GrainGenes Reference Report: GNM-43-931

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Reference	GNM-43-931
Title	Linkage mapping and nucleotide polymorphisms of the 6-SFT gene of cool-season grasses
Journal	Genome
Year	2000
Volume	43
Pages	931-938
Author	Wei JZ Chatterton NJ Larson SR Wang RRC
Abstract	Summary: Fructan plays an important role as an alternate carbohydrate and may contribute to drought and cold-stress tolerances in various plant species. The gene coding for sucrose:fructan 6-fructosyltransferase (6-SFT; EC 2.4.1.10), an enzyme that catalyzes the formation and extension of beta-2,6-linked fructans (levans), is important to fructan synthesis in many cool-season grasses, including cereal species. In this study, we compared a conserved sequence from the 6-SFT gene in barley with comparable sequences in 20 other cool-season grasses. We detected several DNA length polymorphisms, including variations in one simple-sequence repeat (SSR) in a 6-SFT intron of the barley cultivars Steptoe and Morex. Using the 'Steptoe' x 'Morex' doubled-haploid mapping population, the 6-SFT gene was genetically mapped to the distal region in the short arm of barley chromosome 1 (7H), where it is closely linked with trait locus Rpg1. Primers designed from other conserved regions of the barley 6-SFT gene successfully amplified 351- or 354-bp sequences of this gene from diverse cool season grass species. Sequence identities of the PCR products were greater than 80% among the 21 species. Phylogeny, as determined using these DNA sequences, is similar to that obtained from rDNA ITS sequences, and congruent with our current knowledge of genome relationships
Keyword	[ Hide all but 1 of 46 ] barley barley cultivars barley leaves canada carbohydrate cold stress conserved region dna dna sequence doubled haploid drought enzyme extension festuca fructan fructosyltransferase gene genome hordeum vulgare intron its key enzyme linkage linkage mapping locus mapping nucleotide oligomers pcr phleum pratense phylogeny polymorphism population primer products rdna rdna-its relationships rpg1 sequence simple sequence repeat ssr sucrose-fructan 6-fructosyltransferase synthesis tobacco tolerance

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