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GrainGenes Reference Report: GNM-43-698

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Reference	GNM-43-698
Title	Identification and characterization of wheat-wheatgrass translocation lines and localization of barley yellow dwarf virus resistance
Journal	Genome
Year	2000
Volume	43
Pages	698-706
Author	Crasta OR [ Show all 8 ]
Abstract	Stable introgression of agronomically important traits into crop plants through wide crossing often requires the generation and identification of translocation lines. However, the low efficiency of identifying lines containing translocations is a significant limitation in utilizing valuable alien chromatin-derived traits. Selection of putative wheatgrass-wheat translocation lines based on segregation ratios of progeny from gamma-irradiated seed using a standard phenotypic analysis resulted in a low 4% success rate of identifying barley yellow dwarf virus (BYDV) resistant and susceptible translocation lines. However, 58% of the susceptible progeny of this irradiated seed contained a Thinopyrum intermedium chromosome-specific repetitive sequence, which indicated that gamma-irradiation-induced translocations occurred at high rate. Restriction fragment length polymorphism (RFLP) analysis of susceptible lines containing alien chromatin, their resistant sister lines and other resistant lines showed that more than one third of the progeny of gamma-irradiated double monosomic seeds contained wheatgrass-wheat translocations. Genomic in situ hybridization (GISH) analysis of selected lines confirmed that these were wheatgrass-wheat translocation lines. This approach of initially identifying BYDV susceptible deletion lines using an alien chromosome-specific repetitive sequence followed by RFLP analysis of their resistant sister lines efficiently identified resistant translocation lines and localized the BYDV resistance to the distal end of the introgressed Th. intermedium chromosome.
Gene	Bdv2 (Triticum)
Locus	Bdv2
Keyword	[ Hide all but 1 of 46 ] agropyron barley barley yellow dwarf virus barley yellow dwarf virus resistance canada chromatin chromosome chromosome segments deletion lines dna sequence dwarf virus fragment length polymorphism gene introgression generation germplasm specifying resistance gish hybridization in situ in situ hybridization introgression length polymorphism localization molecular biology molecular cytogenetic analysis polymorphism progeny radiation repetitive elements repetitive sequence resistance resistant rflp rflp analysis seed segregation selection sequence streak mosaic-virus thinopyrum thinopyrum intermedium translocation translocation lines virus virus-resistance wide crosses yellow dwarf virus

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