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GrainGenes Reference Report: GNM-43-556

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Reference	GNM-43-556
Title	Molecular analyses of a repetitive DNA sequence in wheat (Triticum aestivum L.)
Journal	Genome
Year	2000
Volume	43
Pages	556-563
Author	Ueng PP [ Show all 8 ]
Abstract	Summary: A repetitive sequence designated WE35 was isolated from wheat genomic DNA. This sequence consists of 320-bp repeat unit and represents approximately 0.002% of the total wheat DNA. It is unidirectionally distributed either continuously or discretely in the genome. Ladder-like banding patterns were observed in Southern blots when the wheat genomic DNA was restricted with endonuclease enzymes EcoRI, HincII, NciI, and NdeI, which is characteristic for tandemly organized sequences. Two DNA fragments in p451 were frequently associated with the WE35 repetitive unit in a majority of lambda wheat genomic clones. A 475-bp fragment homologous to the 5'-end long terminal repeat (LTR) of cereal retroelements was also found in some lambda wheat genomic clones containing the repetitive unit. Physical mapping by fluorescence in situ hybridization (FISH) indicated that one pair of wheat chromosomes could be specifically detected with the WE35 positive probe p551. WE35 can be considered a chromosome-specific repetitive sequence. This repetitive unit could be used as a molecular marker for genetic, phylogenetic, and evolutionary studies in the tribe Triticeae
Keyword	[ Hide all but 1 of 45 ] banding bare-1 barley canada centromere cereal chromosome clone dna dna hybridization dna sequence endonuclease enzyme evolution family fish fluorescence fluorescence in situ hybridization gene location genetic analysis genetic markers genome genomic dna hybridization in situ in situ hybridization long terminal repeat mapping marker molecular marker organization patterns physical mapping repeat repetitive dna repetitive dna sequence repetitive sequence restriction endonuclease analysis retroelements retrotransposon sequence tribe triticeae triticum triticum aestivum

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