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GrainGenes Reference Report: GNM-43-503

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Reference	GNM-43-503
Title	Homoeoallelic gene Ncc-tmp of Triticum timopheevii conferring compatibility with the cytoplasm of Aegilops squarrosa in the tetraploid wheat nuclear background
Journal	Genome
Year	2000
Volume	43
Pages	503-511
Author	Asakura N Nakamura C Ohtsuka I
Abstract	Summary: A nuclear gene, Ncc-tmp1A, of Triticum timopheevii is required for the nucleus-cytoplasm (NC) compatibility in tetraploid NC hybrids with the cytoplasm of Aegilops squarrosa. A euploid NC hybrid of T. durum was previously produced by introgressing the gene from chromosome 1A of T. timopheevii. To examine the possible presence of a functional homoeoallele in the G genome of T. timopheevii, segregation of seed viability was studied as a marker phenotype in BC1S involving the two types of NC hybrids, (Ae. squarrosa)--T. timopheevii and (Ae. squarrosa)--T. turgidum. The result of these test crosses suggested that the G genome possesses a functional homoeoallele Ncc-tmp1G. Segregation of two RAPD (random amplified polymorphic DNA) markers that were closely linked to Ncctmp1A was further studied among the viable BC1S obtained from a test cross of (Ae. squarrosa)--T. timopheevii x T. turgidum. Some viable BC1 segregants without the markers were obtained, suggesting a limited degree of transmission of chromosome 1G carrying Ncc-tm1G. However, a similar RAPD analysis of BC1S obtained after backcrosses of reciprocal F1S of T. timopheevii/T. turgidum with T. turgidum showed random marker segregation. Thus, it was concluded that Ncc-tm1A is not required for compatibility with its own cytoplasm. Southern blot analysis of the euploid NC hybrid using RFLP (restriction fragment length polymorphism) markers on the homoeologous group 1 chromosomes showed that Ncc-tm1A locates in the centromeric region
Keyword	[ Hide all but 1 of 58 ] aegilops aegilops squarrosa alleles alloplasmic durum wheat backcross canada centromere chloroplast dna chromosome chromosome maps compatibility cross cytoplasm dna durum durum wheat evolution fragment length polymorphism gene genetic markers genome group-1 group-1 chromosomes hybrid intergeneric hybridization introgression its length polymorphism linkage marker molecular genetics nc hybrids ncc genes nuclei nucleus-cytoplasm (nc) compatibility phenotypes phylogeny polymorphic polymorphism polyploid wheats random amplified polymorphic dna rapd rapd analysis rflp rflp analysis seed seed set segregation squarrosa tetraploid wheat tetraploidy timopheevii transmission triticum durum triticum timopheevii turgidum vi genes viability

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