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GrainGenes Reference Report: GNM-43-483

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Reference	GNM-43-483
Title	A matrix attachment region is located upstream from the high-molecular weight glutenin geneBx7 in wheat (Triticum aestivum L.)
Journal	Genome
Year	2000
Volume	43
Pages	483-486
Author	Rampitsch C Jordan MC Cloutier S
Abstract	Summary: A 2.2-kb nucleotide sequence rich in AT, located upstream from Bx7 allele of the high-molecular-weight glutenin Glu-B1 locus in wheat (Triticum aestivum cv. Glenlea) was cloned following amplification by PCR. The 5' region of this sequence contains motifs typically found in matrix attachment regions (MARs) in other plants. We have shown that part of the 2.2-kb DNA binds to wheat nuclear matrix (NM) in vitro, at least as strongly as a known MAR (Adh1) from maize suggesting that there is a MAR upstream of Bx7. This MAR is approximately 800 bases in length running from -750 to -1560 bases, relative to the start codon. Although the MAR is associated with a tissue-specific gene and is beside a strong tissue-specific promoter, the MAR sequence did not lead to tissue-specific expression of the beta-glucuronidase marker gene under the control of the rice action promoter in various tissues. Presence of the MAR was only slightly beneficial with respect to expression levels, which were not greatly altered in transient expression assays in various wheat tissues although a slight increase in the number of foci was observed in leaves, which have low transformation efficiencies
Keyword	[ Hide all but 1 of 46 ] alleles amplification assay attachment attachment regions bases beta-glucuronidase binds biolistics canada codon codons dna dna binding motifs endosperm enhancer gene gene expression genetic mapping glutenin high-molecular-weight glutenin in vitro loci locus maize marker matrix attachment region molecular weight nucleotide nucleotide sequence particle bombardment pcr polymerase chain reaction promoter reporter gene rice sequence tissue tissue specific expression transformation transgene expression transgenic plant transient transient expression triticum triticum aestivum

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