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GrainGenes Reference Report: GNM-43-298

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Reference	GNM-43-298
Title	Chromosomal location of AFLP markers in common wheat utilizing nulli-tetrasomic stocks
Journal	Genome
Year	2000
Volume	43
Pages	298-305
Author	Huang XQ [ Show all 6 ]
Abstract	Summary: Amplified fragment length polymorphism (AFLP) markers with a total of 256 EcoRI + ANN - MseI + CNN primer combinations were investigated employing the common wheat cultivar Triticum aestivum 'Chinese Spring.' On average, 103 fragments per primer combination were amplified, ranging from a maximum of 226 fragments to a minimum of 18 fragments. The primer combinations E + AAA - M + CNN and E + ATT - M + CNN produced very few distinct fragments. By using 15 randomly chosen EcoRI + ANN - MseI + CNN primer combinations, 928 AFLP markers were allocated to wheat chromosomes, of which 131 were assigned to specific chromosome arms. These AFLP markers were locus-specific and randomly distributed on the different chromosomes. In addition, 6 and 41 AFLP markers were simultaneously absent in two nulli-tetrasomics (NTs) of both homoeologous and non-homoeologous groups, respectively, whereas additional fragments were detected in N1BT1A, N5AT5D, and N6BT6A lines
External Databases	Pubmed: 10791818
Keyword	[ Hide all but 1 of 29 ] aflp aflp marker aneuploid aneuploidy barley canada chinese spring chromosomal location chromosome assignment chromosome maps chromosomes common wheat diversity fragment length polymorphism gene genetic mapping genetic markers genetic polymorphism inheritance localization loci marker polymorphism qtl resistance rice triticum triticum aestivum wheat cultivar

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