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GrainGenes Reference Report: GNM-42-1242

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Reference	GNM-42-1242
Title	The Ha locus of wheat: Identification of a polymorphic region for tracing grain hardness in crosses
Journal	Genome
Year	1999
Volume	42
Pages	1242-1250
Author	Turner M [ Show all 6 ]
Abstract	Summary: The grain softness proteins or friabilins are known to be composed of three main components: puroindoline a, puroindoline b, and GSP-1. cDNAs for GSP-1 have previously been mapped to group-5 chromosomes and their location on chromosome 5D is closely linked to the grain hardness (Ha) locus of hexaploid wheat. A genomic DNA clone containing the GSP-1 gene (wGSP1-A1) from hexaploid wheat has been identified by fluorescent in situ hybridization as having originated from the distal end of the short arm of chromosome 5A. A genomic clone containing the gene (wGSP1-D1) was also isolated from Aegilops tauschii, the donor of the D genome to bread wheat. There are no introns in the GSP-1 genes, and there is high sequence identity between wGSP1-A1 and wGSP1-D1 up to 1 kb 5' and 300 bp 3' to wGSP1-D1. However, regions further upstream and downstream of wGSP1-D1 share no significant sequence identity to corresponding sequences in wGSP1-A1. These regions therefore identified potentially valuable sequences for tracing the Ha locus through assaying polymorphic DNA sequences. The sequence from 300 to 500 bp 3' to wGSP1-D1 (wGSP1-D13) was mapped to the Ha locus in a mapping population. wGSP1-D13 was also tightly linked to genes for puroindoline a and puroindoline b which have been previously mapped to be at the Ha locus. In addition wGSP1-D13 was used to detect RFLPs between near isogenic soft and hard Falcon lines and in a random selection of soft and hard wheats
External Databases	Pubmed: 10659793 https://doi.org/10.1139/g99-075
Allele	Gsp-D1a (Triticum) Gsp-D1c (Triticum) AF177219
Gene	Gsp-A1 (Triticum) AF177218
Germplasm	CPI 110799
Keyword	[ Hide all but 1 of 54 ] 5a aegilops aegilops tauschii base sequence bread bread wheat cdna chromosome 5a chromosome mapping complementary dna crosses,genetic d genome dna dna hybridization dna plant dna sequence fluorescent in situ hybridization friabilin gene gene location genetic mapping genetic polymorphism genome genome analysis genomic dna genomic library grain hardness hard seeds hard wheat hardness hexaploid hexaploid wheat hybridization in situ in situ hybridization intron loci locus mapping molecular sequence data nucleic acid hybridization polymorphism (genetics) population puroindoline puroindoline-a puroindoline-b rflp selection sequence soft softness softness protein tauschii triticum aestivum

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