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GrainGenes Reference Report: GNM-42-1071

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Reference	GNM-42-1071
Title	Sequence analysis of a rice BAC covering the syntenous barley Rpg1 region
Journal	Genome
Year	1999
Volume	42
Pages	1071-1076
Author	Han F [ Show all 9 ]
Abstract	Summary: In the course of map-based cloning of the barley stem rust resistance gene Rpg1, we identified a rice bacterial artificial chromosome (BAC) containing the Rpg1 flanking markers. Based on the excellent gene order colinearity between barley and rice in this region, we expected that this rice BAC would contain the barley Rpg1 homologue. In order to identify the putative rice homologue, we sequenced ca. 35 kb of the rice BAC at random and then an additional 33 kb of contiguous sequence between the two most closely spaced Rpg1 flanking markers. Sequence analysis revealed a total of 15 putative genes, 5 within the 33-kb contiguous region. A rice Rpg1 homologue was not identified, although a gene encoding a hypothetical polypeptide with similarity to a membrane protein could not be eliminated as a candidate. Surprisingly, four of the genes identified in the 33-kb contiguous rice sequence showed a high degree of similarity with genes on Arabidopsis chromosome 4. The genome regions harboring these genes showed some relatedness, but many rearrangements were also evident. These data suggest that some genes have remained linked even over the long evolutionary separation of Arabidopsis and rice, as has also been reported for mammals and invertebrates
External Databases	Pubmed: 10659772
Keyword	[ Hide all but 1 of 38 ] arabidopsis thaliana artificial chromosome bac barley cell membranes chromosome-4 cloning disease resistance dna libraries flanking markers fungal diseases gene gene order genetic mapping genetic markers genetic resistance genome hordeum vulgare linkage map-based cloning marker membrane oryza sativa polypeptide rearrangements resistance resistance gene rice rpg1 rpg1 region rust rust resistance rust resistance gene separation sequence sequence analysis stem stem rust

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