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GrainGenes Reference Report: GNM-42-1050

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Reference	GNM-42-1050
Title	Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis
Journal	Genome
Year	1999
Volume	42
Pages	1050-1056
Author	Chague V [ Show all 9 ]
Abstract	Summary: Microsatellite and random amplified polymorphic DNA (RAPD) primers were used to identify molecular markers linked to the Yr15 gene which confer resistance to stripe rust (Puccina striiformis Westend) in wheat. By using near isogenic lines (NILs) for the Yr15 gene and a F(2) mapping population derived from crosses of these lines and phenotyped for resistance, we identified one microsatellite marker (GWM33) and one RAPD marker (OPA19(800)) linked to Yr15. Then, bulked segregant analysis was used in addition to the NILs to identify RAPD markers linked to the target gene. Using this approach, two RAPD markers linked to Yr15 were identified, one in coupling (UBC199(700)) and one in repulsion phase (UBC212(1200)). After MAPMAKER linkage analysis on the F(2) population, the two closest markers were shown to be linked to Yr15 within a distance of about 12 cM. The recombination rates were recalculated using the maximum likelihood technique to take into account putative escaped individuals from the stripe rust resistance test and obtain unbiased distance estimates. As a result of this study, the stripe rust resistance gene Yr15 is surrounded by two flanking PCR markers, UBC199(700) and GWM33, at about 5 cM from each side
External Databases	Pubmed: 10659769
Keyword	[ Hide all but 1 of 38 ] bulked segregant analysis chromosome maps disease resistance dna gene genetic mapping genetic markers isogenic lines linkage linkage analysis mapping marker microsatellite microsatellite marker molecular marker near isogenic lines pcr pcr markers phenotypes population primer puccinia striiformis random amplified polymorphic dna rapd rapd markers rates recombination resistance resistance gene rust rust diseases rust resistance rust resistance gene segregant analysis stripe rust stripe rust resistance gene triticum aestivum yr15

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