Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: GNM-39-439

[Submit comment/correction]

Reference	GNM-39-439
Title	Analysis of cereal chromosomes by atomic force microscopy
Journal	Genome
Year	1996
Volume	39
Pages	439-444
Author	McMaster T Winfield M Karp A Miles M
Abstract	Atomic force microscopy has been applied to the study of plant chromosomes from cereal grasses Triticum aestivum (bread wheat), Triticum tauschii, and Hordeum vulgare (barley) Using standard mitotic metaphase squashes, high resolution images have been obtained of untreated chromosomes and also of chromosomes after C-banding, N-banding, and in situ hybridization The true 3-dimensional nature of the images permits detailed analysis of the surface structure and, on untreated uncoated chromosomes, surface features on a length scale consistent with nucleosome structures have been observed C+ and N+ regions are manifest as areas of high relief on a slightly collapsed chromosome structure In situ hybridization leads to a more severe degradation of the native structure, although it is still possible to correlate the optical signal with the topography of the hybridized chromosome.
Keyword	[ Hide all but 1 of 13 ] c-bands chromosome chromosome-analysis chromosome-banding dna hybridization fluorescent-in-situ-hybridization hordeum vulgare imagery microscopy n-bands triticum triticum aestivum triticum tauschii

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.