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GrainGenes Reference Report: GNM-39-288

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Reference
GNM-39-288
Title
Molecular cloning and characterization of an unusually large intergenic spacer from the Nor-B2 locus of hexaploid wheat
Journal
Genome
Year
1996
Volume
39
Pages
288-292
Author
Sardana R
Flavell R
Abstract
An allelic rDNA variant from the Nor-B2 locus of 'Bezostaya' wheat that forms an especially active nucleolus was cloned and characterized It carries an unusually large intergenic spacer compared with rDNA units in most other wheat genotypes The additional intergenic length is in the array of 135-bp A repeats and not in other internal repeats These A repeats have sequences nearly identical to other A repeats described for other alleles It is suggested therefore that the more active Nor-B2 locus of 'Bezostaya' may be due to the constituent rDNA units possessing a larger array of A repeats
Keyword
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cloning
genes
loci
molecular sequence data
nucleolus-organizer
nucleotide sequence
repetitive dna
restriction mapping
ribosomal dna
ribosomal-rna
triticum aestivum

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