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GrainGenes Reference Report: GNM-38-1082

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Reference	GNM-38-1082
Title	Repeated DNA sequences isolated by microdissection. I. Karyotyping of barley (Hordeum vulgare L.)
Journal	Genome
Year	1995
Volume	38
Pages	1082-1090
Author	Busch W Martin R Herrmann R Hohmann U
Abstract	We report on microdissection, cloning and sequence, and Southern and fluorescence in situ hybridization (FISH) analysis of one moderately and one highly amplified repetitive DNA element, pHvMWG2314 and pHvMWG2315, respectively, isolated from barley (Hordeum vulgare L.) chromosome arm 3HL The pHvMWG2315 sequence hybridizes to all 14 telomeric or subtelomeric regions of the barley chromosomes as determined by FISH The 50 different hybridization sites that include intercalary signals allow the discrimination of all 14 chromosome arms and the construction of a karyotype of barley The tandemly repeated subtelomeric element of 331 bp exists in all Triticeae species tested (H vulgare, Agropyron elongatum, Secale cereale, Triticum tauschii, T turgidum, and T aestivum). It is AT rich (66%), exhibits 84% sequence homology to subfragments of the D genome 'specific' 1-kb element pAs1 of T. tauschii and 75% homology to the interspersed genome-specific DNA sequence pHcKB6 from H. chilense. The repetitive sequence pHvMWG2314 is moderately amplified in barley and highly amplified in hexaploid wheat. The in situ experiments revealed no distinct signals on barley chromosomes, indicating a dispersed character for the sequence. The significance of the results for the identification of chromosomes and chromosome aberrations in FISH experiments are discussed.
Keyword	[ Hide all but 1 of 18 ] dna hybridization dna probes elymus elongatus fluorescence fluorescence-in-situ-hybridization hordeum vulgare karyotype molecular sequence data molecular-mapping nucleotide sequence phylogeny repetitive dna secale cereale southern-blotting tirticum-tauschii triticum triticum aestivum triticum turgidum

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