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GrainGenes Reference Report: GNM-38-889

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Reference	GNM-38-889
Title	Refined physical mapping of the Sec-1 locus on the satellite of chromosome 1R of rye (Secale cereale)
Journal	Genome
Year	1995
Volume	38
Pages	889-893
Author	Busch W Herrmann R Martin R
Abstract	The Sec-1 locus (omega-secalin) of rye (Secale cereale L.) was mapped in the satellite of the short arm of chromosome 1R using fluorescence in situ hybridization and a genomic probe called pSec2B Sec-1 is located in the middle of the satellite at the junction of the proximal euchromatic and the distal heterochromatic regions Double hybridization experiments using rDNA and pSec2B showed that the NOR spans over the secondary constriction of the short arm of chromosome 1R and that there is a clearly visible gap between the NOR and Sec-1 Heterologous hybridization of pSec2B to barley visualized the B-hordein locus on chromosome 1H
Keyword	[ Hide all but 1 of 14 ] chromosome dna hybridization dna probes fluorescence-in-situ-hybridization hordein hordeum vulgare loci molecular-mapping nucleolus-organizer prolamin ribosomal dna satellite-dna secale cereale secalin

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