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GrainGenes Reference Report: GNM-38-737

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Reference	GNM-38-737
Title	Chromosome 5D instability in cell lines of Triticum tauschii and morphological variation in regenerated plants
Journal	Genome
Year	1995
Volume	38
Pages	737-742
Author	Winfield M Karp A Lazzeri P Davey M
Abstract	Immature embryos of Triticum tauschii cultured on L2 medium with 30 g.L-1 maltose gave rise to compact and highly structured callus that readily regenerated shoots when transferred to MS medium supplemented with zeatin and indole-3-acetic acid Two cell suspensions were initiated from the callus induced on L2 medium An analysis of chromosome number in these cell suspensions after 3 months of culture showed that 95 and 75% of the cells had a normal complement After 5 months, in both lines the majority of cells had chromosome complements of 2n = 13 and many of these aneuploid cells possessed a dicentric chromosome C-banding indicated that the dicentric was often formed from chromosomes 2D and 5D Fifty-six plants regenerated from callus were grown to maturity. Variation was observed in tiller number, flowering time, and seed-set: in two cases, the variation in early flowering was shown to be heritable. All the regenerants analysed had normal chromosome counts (2n = 2x = 14) and meiotic analyses of 35 plants revealed no obvious structural rearrangements.
Keyword	[ Hide all but 1 of 14 ] aneuploidy c-bands chromosome chromosome-banding chromosome-number dicentric-chormosomes dicentrics embryo-culture flowering-date plant morphology regeneration seed-set shoots triticum

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