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GrainGenes Reference Report: GNM-37-1056

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Reference	GNM-37-1056
Title	The molecular identification of the midgut chromosome from the rye genome
Journal	Genome
Year	1994
Volume	37
Pages	1056-1061
Author	Francki M Langridge P
Abstract	The diminutive 'midget' chromosome is found in plants containing a wheat nuclear genome with a substituted rye cytoplasm This cytoplasmic substituted line arose during successive backcrossing of a wheat/rye amphiploid to wheat as the recurrent male parent Southern and in situ hybridization with a dispersed repeat sequence specific for rye, R173, indicates that the midget chromosome originates from within the rye genome Various DNA markers previously mapped to group I chromosomes of wheat and barley were used to trace the origin of the midget chromosome from within the rye genome Ten short arm and 36 long arm probes were used and one marker was identified, which hybridizes to the midget chromosome and maps to the proximal region of the long arm of chromosome 1R An additional marker was generated from a genomic library of the line containing the midget chromosome. This also maps to the long arm of 1R. The results indicate that the midget chromosome contains a small segment of the long arm of chromosome 1R.
Keyword	[ Hide all but 1 of 17 ] backcrossing chromosome-maps cytoplasm dna hybridization dna probes genetic markers genome-analysis genomes hybrids intergeneric-hybridization microchromosomes polymorphism repetitive dna restriction fragment length polymorphism secale cereale southern-blotting triticum aestivum

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