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GrainGenes Reference Report: GNM-37-910

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Reference	GNM-37-910
Title	The identification of random amplified polymorphic DNA markers for daylength insensitivity in oat
Journal	Genome
Year	1994
Volume	37
Pages	910-914
Author	Wight C Penner G
Abstract	Daylength insensitive accessions of Avena sativa L are being used to develop cultivars that will flower normally when grown under short or long photoperiods Field data indicate that the insensitivity trait is under the control of a single dominant gene, designated Dil The random amplified polymorphic DNA (RAPD) technique and bulk segregant analysis of daylength sensitive and insensitive plants were used to find markers for this gene Five of 200 random decamer primers tested produced polymorphic bands, which were shown to be linked to the trait using 30 homozygous insensitive and 30 homozygous sensitive F3 individuals Three of the markers produced a band in the presence of the dominant allele, and two in its absence. Segregation analysis showed that markers 221 and 136 could be mapped to within 9.8 +/- 4.6 and 13.9 +/- 5.4 cM of the trait, respectively; that is, close enough to be useful in a breeding program. A study of different cultivars suggested that the band produced by primer 136 is actually the more closely linked marker and the only one present in the original Dil gene donor CAV2700. The possibility of using both markers in populations derived from different cultivars is discussed.
Gene	Di-1 (Avena)
Germplasm	OA906-1-16 (oat) Premier (oat)
Locus	Di-1 ubc136 ubc221
Map Data	Oat-1994-PrxOA
Probe	ACOR221 ACOR209 ACOR242 ACOR254 ACOR372
Keyword	[ Hide all but 1 of 11 ] avena sativa di1 gene dominance gene-mapping genes genetic markers linkage-groups photoperiod photoperiod-insensitivity photoperiodism segregation

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