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GrainGenes Reference Report: GNM-37-607

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Reference	GNM-37-607
Title	Genomic in situ hybridization in Avena sativa
Journal	Genome
Year	1994
Volume	37
Pages	607-612
Author	Chen Q Armstrong K
Abstract	Genomic fluorescent in situ hybridization was employed in the study of the genome organization and evolution of hexaploid oat (Avena sativa L cv. Sun II, AACCDD, 2n = 6x = 42). Genomic DNAs from two diploid oat species, Avena strigosa (genomic constitution AsAs, 2n = 14) and Avena pilosa (genomic constitution CpCp, 2n = 14), were used as probes in the study. The DNA from A. strigosa labelled 28 of the 42 (2/3) chromosomes of the hexaploid oat, while 14 of the 42 (1/3) chromosomes were labelled with A. pilosa DNA, indicating a close relationship between the A and D genomes. Results also suggested that at least 18 chromosomes (9 pairs) were involved in intergenomic interchanges between the A and C genomes.
Keyword	[ Hide all but 1 of 15 ] a genome avena nuda avena pilosa avena sativa c genome chromosome-translocation d genome dna hybridization dna probes fluorescein fluorescein-isothiocyanate genome-analysis genomes intergenome-translocation labeling

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