GrainGenes Reference Report: GNM-37-506
Reference
GNM-37-506
Title
A method for detecting DNA polymorphism in large populations
Journal
Genome
Year
1994
Volume
37
Pages
506-508
Author
Aitken S Tinker N Mather D Fortin M
Abstract
Molecular markers linked to loci of interest can be used for fine mapping a particular area of a genome or for marker-assisted selection We present an approach for screening individual plants with polymorphic markers that facilitates phenotyping in large populations Polymorphic DNA fragments, amplified by PCR, are labelled with digoxigenin and used as probes on slot blots of amplified DNA from the individual plants to be tested DNA is obtained by a simple two-tube purification method The colorimetric detection of alleles on the blots is more reliable, and more amenable to automation, than conventional staining of electrophoresis gels
Keyword
[ Hide all but 1 of 11 ] alleles detection digoxigenin dna dna hybridization dna probes genetic markers genetic polymorphism hordeum vulgare labeling plants
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: GNM-37-506
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||