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GrainGenes Reference Report: GNM-37-320

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Reference	GNM-37-320
Title	Amplification of DNA sequences in wheat and its relatives: the Dgas44 and R350 families of repetitive sequences
Journal	Genome
Year	1994
Volume	37
Pages	320-327
Author	McNeil D Lagudah E Hohmann U Appels R
Abstract	The sequence of a Triticum tauschii genomic clone representing a family of D-genome amplified DNA sequences, designated Dgas44, is reported The Dgas44 sequence occurs on all chromosomes of the D genome of wheat, Triticum aestivum, and in situ hybridization revealed it to be evenly dispersed on all seven chromosome pairs An internal HindIII fragment of Dgas44, designated Dgas44-3, defines the highly amplified region that is specific to the D genome The polymerase chain reaction was used to amplify a 236-bp fragment within Dgas44-3 from chromosomes 1D, 2D, 3D, 4D, SD, and 7D, and identical copies of this region of the Dgas44-3 sequence were found among the isolates from each of the chromosomes The Dgas44-3 sequence population from specific chromosomes differed on average by 0.22% from the original Dgas44 sequence. The Dgas44 sequence was found to differentiate between the D genome present in T. aestivum, T. tauschii, hexaploid T. crassum, T. cylindricum, T. ventricosum, in which the sequence was present in a highly amplified form and T. juvenale, T. syriacum, and tetraploid T. crassum where the sequence family was difficult to detect. Another class of amplified sequences previously considered to be rye 'specific,' R350, was isolated from tetraploid wheat and its dispersed distribution on chromosomes was similar to the Dgas44 family in T. tauschii. In contrast with the Dgas44 sequence family, genome specificity for the remnant R350 sequence family was not evident since it was present on all wheat chromosomes.
Keyword	[ Hide all but 1 of 12 ] chromosome d genome dna amplification dna hybridization genome-analysis genomes molecular sequence data nucleotide sequence repetitive dna triticum triticum aestivum wild-plants

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