GrainGenes Reference Report: GNM-36-998
Reference
GNM-36-998
Title
Banding of rye (Secale cereale) chromosomes using the restriction enzymes AluI, DraI, HpaII, and MspI
Journal
Genome
Year
1993
Volume
36
Pages
998-1002
Author
Stober T Gunther T Hesemann C
Abstract
Mitotic metaphase chromosomes of the rye inbred line L 301, which belongs to the sortiment of the University of Hohenheim, were treated in situ with the restriction enzymes AluI (recognition sequence: 5'-AC/GT-3'), DraI (recognition sequence: 5'-TTT/AAA-3'), and the isoschizomeres HpaII and MspI (recognition sequence: 5'-C/CGG-3') and stained with Giemsa The chromosomes indicated similar banding patterns in comparison with the conventional Giemsa-C-banding However, we have found in rye chromosomes after restrictase treatment that the telomeric bands were reduced in extension In a lower degree the centromeric bands of individual chromosomes could be absent in dependence of the used restriction enzymes The number of the intercalary bands were also reduced Nevertheless, the tested restriction enzymes produced characteristic banding patterns of the rye genome This uncomplicated banding technique is suited for a very quick banding method of karyotype analysis especially to obtain a first survey of the band patterns on the rye chromosomes.
Keyword
[ Hide all but 1 of 6 ] chromosome-banding karyotype restriction-banding restriction-endonuclease-analysis restriction-endonucleases secale cereale
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: GNM-36-998
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||