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GrainGenes Reference Report: GNM-36-706

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Reference	GNM-36-706
Title	Identification of the E3900 family, a second family of rye B chromosome specific repeated sequences
Journal	Genome
Year	1993
Volume	36
Pages	706-711
Author	Blunden R [ Show all 7 ]
Abstract	A second family of highly repeated sequences has been identified on the B chromosome of rye (Secale cereale) The E3900 family was detected as a variant band in EcoRI digests of +B DNA. A clone of the basic repeat of the family was obtained, and the organization of the family was investigated by genomic hybridization. The E3900 family has no apparent homology to the A chromosome sequences of rye or other members of the Gramineae. The family has been localized by in situ hybridization to the end of the long arm of the rye B chromosome. The previously characterized E1100 sequence shows in situ hybridization to the same location as the E3900 family. These results are discussed in light of current theories of the origin of B chromosomes.
Keyword	[ Hide all but 1 of 6 ] b-chromosomes dna hybridization in situ hybridization molecular-mapping repetitive dna secale cereale

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