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GrainGenes Reference Report: GNE-156-129

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Reference	GNE-156-129
Title	The presence of tRNA pseudogenes in mammalia and plants and their absence in yeast may account for different specificities of pre-tRNA processing enzymes
Journal	Gene
Year	1995
Volume	156
Pages	129-132
Author	Kacar Y Beier H Gross H
Abstract	Six of 13 cloned members of the human tRNAVal gene family code for tRNAVal pseudogenes, of which all but one are transcribed efficiently in HeLa cell extracts Due to single or multiple mismatches in stem regions, the corresponding pre-tRNAs are resistant against the action of human 5'- and 3'-processing enzymes and are thus prevented from being converted to mature tRNAs Surprisingly, all of them are accurately and efficiently processed to mature-sized tRNA in yeast nuclear extract This is in agreement with corresponding studies of plant pre-tRNAs which are not processed in wheat germ extract but are rapidly processed in yeast extract These observations imply that the yeast pre-tRNA 5' and 3'-maturases do not monitor the three-dimensional structure of their substrates as stringently as mammalian and plant enzymes possibly because tRNA pseudogenes do not occur in yeast.
Keyword	[ Hide all but 1 of 14 ] gene expression hela-cells in-vitro-transcription nucleases plant extracts pre-transfer-rna-maturase pseudogenes saccharomyces cerevisiae transcription transfer-rna triticum aestivum valine wheat germ yeast-extracts

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