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GrainGenes Reference Report: GNE-70-271

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Reference	GNE-70-271
Title	Nucleotide sequence and endosperm-specific expression of the structural gene for the toxin alpha hordothionin in barley Hordeum-vulgare L.
Journal	Gene
Year	1988
Volume	70
Pages	271-281
Author	Rodriguez-Palenzuela P Pintor-Toro JA Carbonero P Garcia-Olmedo F
Abstract	A barley genomic library, obtained by cloning in the vector .lambda. EMBL-4, was screened with a cDNA probe encoding the .alpha.- hordothionin toxin. A positive clone, designated .lambda. TH1, was selected for further characterization. The coding and flanking regions of the .alpha.-hordothionin gene (Hth-1) were sequenced. Hth-1 has two introns of 420 and 91 nucleotides (nt), respectively. The promoter region has the following main features: one TATA box; three CATC boxes; an enhancer-like sequence, starting at nt position .sbd.282 from the first ATG codon, which is homologous to sequences appearing at similar positions in other endosperm genes; two versions of an 18-nt sequence that is more highly repeated in structural domains of several prolamin genes; two extensive regions close to the first ATG codon that are homologous to a sequence located much further upstream in the B-hordein promoter. The transcription start point was determined at nt positions .sbd.46 to .sbd.47, both by the S1 nuclease-protection and by the primer- extension assays. A maximum of 2.sbd.4 copies of the Hth-1 gene per haploid genome was determined by Southern-blot hybridization. Expression of the Hth-1 gene was detected during the cell proliferation stage of endosperm development (maximum at 13.sbd.16 days after pollinization) and was not detected in either etiolated or green coleoptiles.
Probe	pTHG1
Keyword	[ Hide all but 1 of 9 ] Amino acid sequence Coding region DNA sequence Endosperm development Flanking region Genomic DNA clones Molecular sequence data Promoter sequence Transcriptional start sequence

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