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GrainGenes Reference Report: GEN-168-701

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Reference	GEN-168-701
Title	A Chromosome Bin Map of 16,000 Expressed Sequence Tag Loci and Distribution of Genes Among the Three Genomes of Polyploid Wheat
Journal	Genetics
Year	2004
Volume	168
Pages	701-712
Author	[ Hide all but 1 of 47 ] Qi LL Echalier B Chao S Lazo GR Butler GE Anderson OD Akhunov ED Dvorak J Linkiewicz AM Ratnasiri A Dubcovsky J Bermudez-Kandianis CE Greene RA Kantety R La Rota CM Munkvold JD Sorrells SF Sorrells ME Dilbirligi M Sidhu D Erayman M Randhawa HS Sandhu D Bondareva SN Gill KS Mahmoud AA Ma XF Miftahudin Gustafson JP Conley EJ Nduati V Gonzalez-Hernandez JL Anderson JA Peng JH Lapitan NLV Hossain KG Kalavacharla V Kianian SF Pathan MS Zhang DS Nguyen HT Choi DW Fenton RD Close TJ McGuire PE Qualset CO Gill BS
Abstract	Because of the huge size of the common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) genome of 17,300 Mb, sequencing and mapping of the expressed portion is a logical first step for gene discovery. Here we report mapping of 7104 expressed sequence tag (EST) unigenes by Southern hybridization into a chromosome bin map using a set of wheat aneuploids and deletion stocks. Each EST detected a mean of 4.8 restriction fragments and 2.8 loci. More loci were mapped in the B genome (5774) than in the A (5173) or D (5146) genomes. The EST density was significantly higher for the D genome than for the A or B. In general, EST density increased relative to the physical distance from the centromere. The majority of EST-dense regions are in the distal parts of chromosomes. Most of the agronomically important genes are located in EST-dense regions. The chromosome bin map of ESTs is a unique resource for SNP analysis, comparative mapping, structural and functional analysis, and polyploid evolution, as well as providing a framework for constructing a sequence-ready, BAC-contig map of the wheat genome
External Databases	Pubmed: 15514046
Keyword	chromosomes [ Show all 9 ]

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