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GrainGenes Reference Report: GEN-168-687

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Reference
GEN-168-687
Title
A Chromosome Bin Map of 2148 Expressed Sequence Tag Loci of Wheat Homoeologous Group 7
Journal
Genetics
Year
2004
Volume
168
Pages
687-699
Author
[ Hide all but 1 of 46 ]
Hossain KG
Kalavacharla V
Lazo GR
Hegstad J
Wentz MJ
Kianian PMA
Simons K
Gehlhar S
Rust JL
Syamala RR
Obeori K
Bhamidimarri S
Karunadharma P
Chao S
Anderson OD
Qi LL
Echalier B
Gill BS
Linkiewicz AM
Ratnasiri A
Dubcovsky J
Akhunov ED
Dvorak J
Miftahudin
Ross K
Gustafson JP
Radhawa HS
Dilbirligi M
Gill KS
Peng JH
Lapitan NLV
Greene RA
Bermudez-Kandianis CE
Sorrells ME
Feril O
Pathan MS
Nguyen HT
Gonzalez-Hernandez JL
Conley EJ
Anderson JA
Choi DW
Fenton D
Close TJ
McGuire PE
Qualset CO
Kianian SF
Abstract
The objectives of this study were to develop a high-density chromosome bin map of homoeologous group 7 in hexaploid wheat (Triticum aestivum L.), to identify gene distribution in these chromosomes, and to perform comparative studies of wheat with rice and barley. We mapped 2148 loci from 919 EST clones onto group 7 chromosomes of wheat. In the majority of cases the numbers of loci were significantly lower in the centromeric regions and tended to increase in the distal regions. The level of duplicated loci in this group was 24% with most of these loci being localized toward the distal regions. One hundred nineteen EST probes that hybridized to three fragments and mapped to the three group 7 chromosomes were designated landmark probes and were used to construct a consensus homoeologous group 7 map. An additional 49 probes that mapped to 7AS, 7DS, and the ancestral translocated segment involving 7BS also were designated landmarks. Landmark probe orders and comparative maps of wheat, rice, and barley were produced on the basis of corresponding rice BAC/PAC and genetic markers that mapped on chromosomes 6 and 8 of rice. Identification of landmark ESTs and development of consensus maps may provide a framework of conserved coding regions predating the evolution of wheat genomes
External Databases
Pubmed: 15514045
Keyword
barley
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