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GrainGenes Reference Report: GEN-167-439

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Reference	GEN-167-439
Title	Genetics of barley Hooded suppression
Journal	Genetics
Year	2004
Volume	167
Pages	439-448
Author	Roig C [ Show all 9 ]
Abstract	The molecular basis of the barley dominant Hooded (K) mutant is a duplication of 305 bp in intron IV of the homeobox gene Bkn3. A chemical mutagenesis screen was carried out to identify genetical factors that participate in Bkn3 intron-mediated gene regulation. Plants from recurrently mutagenized KK seeds were examined for the suppression of the hooded awn phenotype induced by the K allele and, in total, 41 suK (suppressor of K) recessive mutants were identified. Complementation tests established the existence of five suK loci, and alleles suKB-4, suKC-33, suKD-25, suKE-74, and suKF-76 were studied in detail. All K-suppressed mutants showed a short-awn phenotype. The suK loci have been mapped by bulked segregant analysis nested in a standard mapping procedure based on AFLP markers. K suppressor loci suKB, B, E, and F all map in a short interval of chromosome 7H, while the locus suKD is assigned to chromosome 5H. A complementation test between the four suK mutants mapping on chromosome 7H and the short-awn mutant lks2, located nearby, excluded the allelism between suK loci and lks2. The last experiment made clear that the short-awn phenotype of suK mutants is due to a specific dominant function of the K allele, a function that is independent from the control on hood formation. The suK loci are discussed as candidate participants in the regulation of Bkn3 expression
External Databases	Pubmed: 15166167
Keyword	[ Hide all but 1 of 33 ] aflp aflp marker allelism alters leaf morphology arabidopsis barley bulked segregant analysis chromosome control developmental mutants duplication ectopic expression existence factor gene gene regulation homeobox gene homeodomain proteins identify interval intron knotted class maize map mapping molecular-basis mutagenesis phenotype regulation specific suppression transcriptional regulation transgenic tobacco

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