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GrainGenes Reference Report: GEN-167-343

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Reference	GEN-167-343
Title	A physically anchored genetic map and linkage to avirulence reveals recombination suppression over the proximal region of hessian fly chromosome A2
Journal	Genetics
Year	2004
Volume	167
Pages	343-355
Author	Behura SK [ Show all 6 ]
Abstract	Resistance in wheat (Triticum aestivum) to the Hessian fly (Mayetiola destructor), a major insect pest of wheat, is based on a gene-for-gene interaction. Close linkage (3 plus or minus 2 cM) was discovered between Hessian fly avirulence genes vH3 and vH5. Bulked segregant analysis revealed two DNA markers (28-178 and 23-201) within 10 cM of these loci and only 3 plus or minus 2 cM apart. However, 28-178 was located in the middle of the short arm of Hessian fly chromosome A2 whereas 23-201 was located in the middle of the long arm of chromosome A2, suggesting the presence of severe recombination suppression over its proximal region. To further test that possibility, an AFLP-based genetic map of the Hessian fly genome was constructed. Fluorescence in situ hybridization of 20 markers on the genetic map to the polytene chromosomes of the Hessian fly indicated good correspondence between the linkage groups and the four Hessian fly chromosomes. The physically anchored genetic map is the first of any gall midge species. The proximal region of mitotic chromosome A2 makes up 30% of its length but corresponded to <3% of the chromosome A2 genetic map
External Databases	Pubmed: 15166159
Keyword	[ Hide all but 1 of 37 ] avirulence avirulence gene avirulence genes bulked segregant analysis cecidomyiidae cm cytogenetics dna drosophila fluorescence fluorescence in situ hybridization gene genome hessian fly hybridization in situ in situ hybridization inheritance insect linkage linkage groups locus long arm map mayetiola destructor mayetiola destructor diptera midge oryza wood-mason pest population recombination resistance resistance gene reveal rice gall midge suppression triticum

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