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GrainGenes Reference Report: GEN-43-689

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Reference	GEN-43-689
Title	Isolation and mapping of microsatellite markers specific for the D genome of bread wheat
Journal	Genome
Year	2000
Volume	43
Pages	689-697
Author	Ganal M Roeder M
Type	Article
Language	English
Abstract	The potential of Aegilops tauschii, the diploid progenitor of the D genome of wheat, as a source of microsatellite markers for hexaploid bread wheat was investigated. By screening lambda phage and plasmid libraries of Ae. tauschii genomic DNA, dinucleotide microsatellites containing GA and GT motifs were isolated and a total of 65 functional microsatellite markers were developed. All primer pairs that were functional in Ae. tauschii amplified well in hexaploid wheat. Fifty-five loci amplified by 48 primer sets were placed onto a genetic framework map of the reference population of the International Triticeae Mapping Initiative (ITMI) 'Opata 85' X 'W7984'. The majority of microsatellite markers could be assigned to the chromosomes of the D genome of wheat. The distribution of the markers along the chromosomes is random. Chromosomal location of 22 loci nonpolymorphic in the reference population was determined using nullitetrasomic lines of Triticum aestivum 'Chinese Spring'. The results of this study demonstrate the value of microsatellite markers isolated from Ae. tauschii for the study of bread wheat. The microsatellite markers developed improve the existing wheat microsatellite map and can be used in a wide range of genetic studies and breeding programs.
Keyword	[ Hide all but 1 of 21 ] aegilops tauschii aegilops tauschii ssp. tauschii agronomy center of origin d genome disomic addition line identification endemism evolution and adaptation genetic distance genetic diversity genetic mapping homeology macrosatellites methods and techniques microsatellite marker microsatellites molecular genetics simple sequence repeat ssr triticum aestivum wheat microsatellites

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