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GrainGenes Reference Report: GCE-49-145

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Reference	GCE-49-145
Title	RFLP analysis of Aegilops species belonging to the Sitopsis section
Journal	Genetic Resources and Crop Evolution
Year	2002
Volume	49
Pages	145-151
Author	Giorgi D D'Ovidio R Tanzarella OA Porceddu E
Abstract	Summary: The phylogenetic relationships among the Aegilops species belonging to the Sitopsis section were investigated using RFLP (restriction-fragment-length polymorphism) analysis. Twenty-five probes, each of which hybridised to one or more restriction fragments located in the B-genome chromosomes of cultivated wheats, were used. At least one and in most cases two fragments were located in every B genome chromosome arm. A dendrogram derived from a cluster analysis of the complete RFLP data set showed a subdivision of the species belonging to the Sitopsis section into one group comprising the species of the Truncata subsection and another group comprised of the species of the Emarginata subsection. Dendrograms also were produced using RFLP data from loci located in different combinations of only three chromosomes, and some of these showed different subdivisions of the species. This demonstrates the importance in obtaining reliable classification data of using probes that detect loci evenly distributed in the genome and located in each chromosome arm
Keyword	[ Hide all but 1 of 33 ] aegilops b genome chromosome chromosome arm classification cluster cluster analysis combinations common wheat data set diversity dna fragments genome loci phylogenesis phylogenetic relationship phylogeny polymorphism polyploid wheats probes relationships repeated nucleotide-sequences restriction restriction fragment length rflp rflp analysis sitopsis standard karyotype subdivision triticum wheat wheat evolution

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