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GrainGenes Reference Report: GCE-49-133

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Reference	GCE-49-133
Title	Ecogeographic adaptation and genetic variation in wild barley, Application of molecular markers targeted to environmentally regulated genes
Journal	Genetic Resources and Crop Evolution
Year	2002
Volume	49
Pages	133-144
Author	Liviero L Maestri E Gulli M Nevo E Marmiroli N
Abstract	Summary: Long primer PCR (LP-PCR) markers specifically targeted to sequences involved in the response to abiotic stress were utilised to analyse genetic variation within and among wild barley populations from Israel. Populations were sampled in locations reflecting a wide range of habitats and the potential correlation between genetic variation and ecogeographic parameters was examined. A high level of polymorphism was observed. Most of the variability was due to differences within populations, however a substantial portion of genetic variation (36%) could be attributed to differences among populations, indicating local adaptation of genotypes to microclimatic conditions. Moreover, clustering and ordination techniques showed that genotypes grouped together according to their area of origin and were separated on the basis of ecogeography, chiefly by a combination of temperature, altitude and rainfall variables. Finally, 28% of the polymorphic PCR fragments were significantly correlated with ecogeographic parameters. Our results demonstrate the effectiveness of PCR-based molecular markers targeted to environmentally regulated genes in detecting 'useful' variation and thus in monitoring the impact exerted by adaptation to the environment on genetic differentiation
Keyword	[ Hide all but 1 of 48 ] abiotic stress adaptation altitude application average heterozygosity barley correlation differentiation diversity dna ecogeographic environment fragments gene genetic differentiation genetic variation genotype habitat high-level hordeum vulgare l impact israel long primer pcr marker molecular marker monitoring ordination origin parameters pcr polymorphic polymorphism population primer quantitative trait loci rainfall rapd regulated gene sequence spontaneum stress stress tolerance stress-responsive genes temperature variability wild wild barley winterhardiness

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