Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: AJB-88-1863

[Submit comment/correction]

Reference	AJB-88-1863
Title	Origin and genetic diversity of Spartina anglica (Poaceae) using nuclear DNA markers
Journal	American Journal of Botany
Year	2001
Volume	88
Pages	1863-1867
Author	Ayres DR Strong DR
Abstract	Summary: Spartina alterniflora, introduced into the UK in the 1800s, was the seed parent in an interspecific hybridization with S. maritima. The sterile F1 hybrid S. xtownsendii gave rise to the fertile allopolyploid S. anglica by chromosomal doubling. Previous chromosome, isozyme, and cpDNA surveys did not reveal notable genetic variation within either the parental or the hybrid species. We used nuclear DNA markers (random amplified polymorphic DNA ([RAPD]) and inter-simple sequence repeats (ISSR) to further explore the origin, diversity, and parentage of S. anglica. We found DNA fragments in S. xtownsendii were the aggregate of diagnostic DNA fragments from S. maritima and S. alterniflora, thus confirming its hybrid origin. The S. xtownsendii genotype was identical to most of the S. anglica individuals analyzed, establishing the genetic concordance of these two taxa. We found widespread genetic variation within S. anglica. This could indicate that S. anglica arose several times, from different S. maritima sires. Alternatively, alleles could have been lost through recombination and/or through loss of entire chromosomes in S. anglica. Finally, all but one S. anglica individual had a S. alterniflora component that was indistinguishable from a S. alterniflora plant extant in Marchwood, UK, leaving open the possibility that this plant is the actual seed parent of S. anglica
Keyword	[ Hide all but 1 of 50 ] alleles allopolyploid alterniflora amplified polymorphic dna anglica california chromosome component cpdna diagnostic diversity dna dna fragment dna marker evolution fertile fragments genetic diversity genetic variation genotype gramineae hybrid hybridization intersimple sequence repeat interspecific hybridization isozyme issr loss marker nuclear dna origin parentage poaceae polymorphic polymorphic dna random amplified polymorphic dna rapd recombination repeat san francisco bay seed sequence spartina spartina alterniflora spartina anglica spartina maritima spartina townsendii surveys taxa uk

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.