GrainGenes Reference Report: FMS-178-283
Reference
FMS-178-283
Title
Presence of 16S rRNA genes in multiple replicons in Azospirillum brasilense
Journal
FEMS Microbiology Letters
Year
1999
Volume
178
Pages
283-288
Author
Caballero-Mellado J Lopez-Reyes L Bustillos C
Abstract
Rhizosphere and endophytic Azospirillum brasilense isolates recovered from sugarcane plants and the reference strains Sp7 and Cd were analyzed for plasmid occurrence. All of the 26 A. brasilense isolates analyzed harbored from five to eight replicons. Several strains contained small plasmids from 45 to 70 kb, but all of the isolates harbored other plasmids ranging from 100 to 290 kb and two megareplicons of approximately 1700 and over 1800 kb. Most of the strains contained a replicon with a size of either 570 or 630 kb, and another large 910- or 980-kb replicon. The 1700-kb megareplicon and some others around 600 kb strongly hybridized to 16S rDNA genes, while the 910- or 980-kb replicons hybridized only slightly. This suggests that the A. brasilense genome is composed of multiple minichromosomes instead of a single circular chromosome. The apparent genome complexity of A. brasilense deserves to eventually be resolved by complete genome sequencing
Keyword
[ Hide all but 1 of 11 ] chromosome endophyte gene genome plasmids rdna rhizosphere rrna genes saccharum officinarum sp7 sugarcane
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GrainGenes Reference Report: FMS-178-283
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||