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GrainGenes Reference Report: EJP-108-449

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Reference	EJP-108-449
Title	Dominant colonisation of wheat roots by Pseudomonas fluorescens Pf29A and selection of the indigenous microflora in the presence of the take-all fungus
Journal	European Journal of Plant Pathology
Year	2000
Volume	108
Pages	449-459
Author	Chapon A Guillerm AY Delalande L Labreton L Sarniguet A
Abstract	Summary: Increases in populations of fluorescent pseudomonads on wheat roots are usually associated with take-all decline, natural control of take-all, a disease caused by the fungus Gaeumannomyces graminis var. tritici (Ggt). Colonisation by Pseudomonas fluorescens strain Pf29A was assessed on the roots of healthy plants and of plants with take- all, and the effect of this bacterium on indigenous populations of fluorescent pseudomonads was studied. The efficacy of Pf29A as an agent for the biocontrol of take-all on five-week-old wheat seedlings was tested in non-sterile conducive soil in a growth chamber. RAPD (random amplification of polymorphic DNA) fingerprinting with a decamer primer was used to monitor strain Pf29A and culturable indigenous rhizoplane populations of fluorescent pseudomonad. Pf29A decreased disease severity and accounted for 44.6% of the culturable fluorescent pseudomonads on healthy plant rhizoplane and 75.8% on diseased plant rhizoplane. Fewer RAPD patterns were obtained when Pf29A was introduced into the soil with Ggt. In the presence of Ggt and necrotic roots, Pf29A became the dominant root coloniser and dramatically changed the diversity and the structure of indigenous fluorescent pseudomonad populations. The results show that Ggt and reduced lesion size on roots can trigger a specific increase in antagonist populations and that the introduction of a biocontrol agent in soil influences the structure of indigenous bacterial populations
Keyword	[ Hide all but 1 of 42 ] amplification bacteria bacterial biocontrol biocontrol agent biological control biological control agents colonization control culturable disease diversity dna dna fingerprinting efficacy fluorescent pseudomonads fungal antagonists fungus gaeumannomyces graminis gaeumannomyces graminis var graminis growth increase indigenous microbial flora microflora pseudomonas pseudomonas fluorescens random amplified polymorphic dna rapd rhizoplane rhizosphere seedling selection soil strain take-all take-all fungus tritici triticum aestivum wheat roots wheat seedlings

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